nutrimorph.py

# Copyright CNRS/Inria/UNS
# Contributor(s): Eric Debreuve (since 2019), Morgane Nadal (2020)
#
# eric.debreuve@cnrs.fr
#
# This software is governed by the CeCILL  license under French law and
# abiding by the rules of distribution of free software.  You can  use,
# modify and/ or redistribute the software under the terms of the CeCILL
# license as circulated by CEA, CNRS and INRIA at the following URL
# "http://www.cecill.info".
#
# As a counterpart to the access to the source code and  rights to copy,
# modify and redistribute granted by the license, users are provided only
# with a limited warranty  and the software's author,  the holder of the
# economic rights,  and the successive licensors  have only  limited
# liability.
#
# In this respect, the user's attention is drawn to the risks associated
# with loading,  using,  modifying and/or developing or reproducing the
# software by the user in light of its specific status of free software,
# that may mean  that it is complicated to manipulate,  and  that  also
# therefore means  that it is reserved for developers  and  experienced
# professionals having in-depth computer knowledge. Users are therefore
# encouraged to load and test the software's suitability as regards their
# requirements in conditions enabling the security of their systems and/or
# data to be ensured and,  more generally, to use and operate it in the
# same conditions as regards security.
#
# The fact that you are presently reading this means that you have had
# knowledge of the CeCILL license and that you accept its terms.

# Time profiling:
#     python -m cProfile -o runtime/profiling.log -s name main.py
# Memory profiling:
#     python -m memory_profiler main.py
#     or
#     mprof run main.py
#     mprof plot

import brick.component.connection as cn_
import brick.component.extension as xt_
import brick.component.soma as sm_
import brick.general.feedback as fb_
import brick.processing.input as in_
import brick.processing.plot as po_
# import brick.processing.image_verification as iv_
# from main_prm import *
from sklgraph.skl_fgraph import skl_graph_t
from sklgraph.skl_graph import plot_mode_e
from sklgraph.skl_map import skl_map_t

import os as os_
import sys as sy_
import time as tm_
# import tracemalloc as tr_

import matplotlib.pyplot as pl_
import numpy as np_
import skimage.io as io_
# from skimage.segmentation import relabel_sequential
import skimage.morphology as mp_
import skimage.measure as ms_
import networkx as nx_


print(sy_.argv, sy_.argv.__len__())

if sy_.argv.__len__() < 2:
    print("Missing parameter file argument")
    sy_.exit(0)
if not (os_.path.isfile(sy_.argv[1]) and os_.access(sy_.argv[1], os_.R_OK)):
    print("Wrong parameter file path or parameter file unreadable")
    sy_.exit(0)


data_path = None
channel = None
size_voxel_in_micron = None
soma_low_c = None
soma_high_c = None
soma_selem_micron_c = None
soma_min_area_c = None
ext_low_c = None
ext_high_c = None
ext_selem_micron_c = None
ext_min_area_c = None
max_straight_sq_dist_c = None
max_weighted_length_c = None
scale_range = None
scale_step = None
alpha = None
beta = None
frangi_c = None
bright_on_dark = None
method = None
with_plot = None
in_parallel = None


exec(open(sy_.argv[1]).read())  # Only with parameters.py (older versions) or search_parameters.py (stable version)

soma_t = sm_.soma_t
extension_t = xt_.extension_t

print(f"STARTED: {tm_.strftime('%a, %b %d %Y @ %H:%M:%S')}")
start_time = tm_.time()

# tr_.start()


# --- Images
# Find the dimension of the image voxels in micron
size_voxel_in_micron = in_.FindVoxelDimensionInMicron(data_path, size_voxel_in_micron=size_voxel_in_micron)

image = io_.imread(data_path)

# Image size verification - simple version without user interface
image = in_.ImageVerification(image, channel)
# iv_.image_verification(image, channel)  # -> PySide2 interface  # TODO: must return the modified image!
# /!\ conflicts between some versions of PySide2 and Python3

image = image[:, 800:, 800:]  # 512 # 562 # Just for development
img_shape = image.shape
# pl_.matshow(image[image.shape[0] // 2, :, :])
# pl_.show()
#
# max_img = image.max()
# image.fill(image.min())
# image[2:3,10:11,:] = max_img
#
print(f"IMAGE: s.{img_shape} t.{image.dtype} m.{image.min()} M.{image.max()}")

# Intensity relative normalization (between 0 and 1).
# TODO: change Frangi parameters to take the normalization btw 0 and 1 into account ?
# image_for_soma = in_.IntensityNormalizedImage(image)
# image_for_ext = in_.IntensityNormalizedImage(image)

# This normalization is essential for the detection of extension and the connexions to the somas!
image_for_soma = in_.NormalizedImage(image)
image_for_ext = in_.NormalizedImage(image)

print(f"NRM-IMG: t.{image_for_soma.dtype} m.{image_for_soma.min():.2f} M.{image_for_soma.max():.2f}")

# --- Initialization
som_nfo = {}
ext_nfo = {}
axes = {}

# n_somas = 0
# n_extensions = 0
# somas = None  # Tuple of soma objects
# extensions = None  # Tuple of extension objects


# --- Somas
print("\n--- Soma Detection")

soma_min_area_c = in_.ToPixel(soma_min_area_c, size_voxel_in_micron, dimension=(0, 1))
soma_selem_c = mp_.disk(in_.ToPixel(soma_selem_micron_c, size_voxel_in_micron))

som_nfo["map"] = soma_t.Map(image_for_soma, soma_low_c, soma_high_c, soma_selem_c)
som_nfo["map"], som_lmp = soma_t.FilteredMap(som_nfo["map"], soma_min_area_c)
som_nfo["lmp"], n_somas = ms_.label(som_nfo["map"], return_num=True)

# som_nfo["lmp"] = relabel_sequential(som_lmp)[0]  # Use relabel instead of label to optimize the algorithm.
# n_somas = som_nfo["lmp"].max()

som_nfo["dist_to_closest"], som_nfo["influence_map"] = soma_t.InfluenceMaps(
    som_nfo["lmp"]
)

somas = tuple(
    soma_t().FromMap(som_nfo["lmp"], uid) for uid in range(1, n_somas + 1)
)

print(f"    n = {n_somas}")
elapsed_time = tm_.gmtime(tm_.time() - start_time)
print(f"\nElapsed Time={tm_.strftime('%Hh %Mm %Ss', elapsed_time)}")

if with_plot:
    fb_.PlotSomas(somas, som_nfo, axes)

# -- Extentions
print("\n--- Extension Detection")

ext_min_area_c = in_.ToPixel(ext_min_area_c, size_voxel_in_micron, dimension=(0, 1))
ext_selem_pixel_c = mp_.disk(in_.ToPixel(ext_selem_micron_c, size_voxel_in_micron))

scale_range_pixel = []
for value in scale_range:
    value_in_pixel = in_.ToPixel(value, size_voxel_in_micron, decimals=1)
    scale_range_pixel.append(value_in_pixel)
scale_range = tuple(scale_range_pixel)

scale_step = in_.ToPixel(scale_step, size_voxel_in_micron, decimals=1)
alpha = in_.ToPixel(alpha, size_voxel_in_micron, decimals=1)
beta = in_.ToPixel(beta, size_voxel_in_micron, decimals=1)
frangi_c = in_.ToPixel(frangi_c, size_voxel_in_micron)

enhanced_ext, ext_scales = extension_t.EnhancedForDetection(
    image_for_ext,
    scale_range,
    scale_step,
    alpha,
    beta,
    frangi_c,
    bright_on_dark,
    method,
    in_parallel=in_parallel)
ext_nfo["coarse_map"] = extension_t.CoarseMap(enhanced_ext, ext_low_c, ext_high_c, ext_selem_pixel_c)
ext_nfo["coarse_map"], ext_lmp = extension_t.FilteredCoarseMap(ext_nfo["coarse_map"], ext_min_area_c)
ext_nfo["map"] = extension_t.FineMapFromCoarseMap(ext_nfo["coarse_map"])
ext_nfo["map"][som_nfo["map"] > 0] = 0
ext_nfo["lmp"], n_extensions = ms_.label(ext_nfo["map"], return_num=True)

# Use relabel instead of label to optimize the algorithm. BUT PROBLEM WITH THE NUMBER OF EXTENSIONS DETECTED !
# ext_nfo["lmp"] = relabel_sequential(ext_lmp)[0]
# n_extensions = ext_nfo["lmp"].max()

extensions = tuple(
    extension_t().FromMap(ext_nfo["lmp"], ext_scales, uid)
    for uid in range(1, n_extensions + 1)
)

print(f"    n = {n_extensions}")
elapsed_time = tm_.gmtime(tm_.time() - start_time)
print(f"\nElapsed Time={tm_.strftime('%Hh %Mm %Ss', elapsed_time)}")
if with_plot:
    fb_.PlotExtensions(extensions, ext_nfo, img_shape)

# -- Preparation for Connections
dijkstra_costs = in_.DijkstraCosts(image, som_nfo["map"], ext_nfo["map"])

# -- Soma-Extention
print("\n--- Soma <-> Extension")

max_straight_sq_dist_c = in_.ToPixel(max_straight_sq_dist_c, size_voxel_in_micron)
max_weighted_length_c = in_.ToPixel(max_weighted_length_c, size_voxel_in_micron)

candidate_conn_nfo = cn_.CandidateConnections(
    somas,
    som_nfo["influence_map"],
    som_nfo["dist_to_closest"],
    extensions,
    max_straight_sq_dist_c,
)

for ep_idx, soma, extension, end_point in candidate_conn_nfo:
    if extension.is_unconnected:
        print(f"    Soma.{soma.uid} <-?-> Ext.{extension.uid}({ep_idx})", end="")
        path, length = cn_.ShortestPathFromToN(
            end_point,
            dijkstra_costs,
            soma.ContourPointsCloseTo,
            max_straight_sq_dist=max_straight_sq_dist_c,
        )
        if length <= max_weighted_length_c:
            cn_.ValidateConnection(soma, extension, path, dijkstra_costs)
            print(": Made")
        else:
            print("")

# for soma in somas:
#     soma.Extend(extensions, som_nfo["dist_to_closest"], dijkstra_costs)

fb_.PrintConnectedExtensions(extensions)
elapsed_time = tm_.gmtime(tm_.time() - start_time)
print(f"\nElapsed Time={tm_.strftime('%Hh %Mm %Ss', elapsed_time)}")
if with_plot:
    fb_.PlotSomasWithExtensions(somas, som_nfo, "all")

# -- Extention-Extention
print("\n--- Extension <-> Extension")

should_look_for_connections = True
while should_look_for_connections:
    som_nfo["soma_w_ext_lmp"] = soma_t.SomasLMap(somas)
    som_nfo["dist_to_closest"], som_nfo["influence_map"] = soma_t.InfluenceMaps(
        som_nfo["soma_w_ext_lmp"]
    )

    candidate_conn_nfo = cn_.CandidateConnections(
        somas,
        som_nfo["influence_map"],
        som_nfo["dist_to_closest"],
        extensions,
        max_straight_sq_dist_c,
    )

    should_look_for_connections = False
    for ep_idx, soma, extension, end_point in candidate_conn_nfo:
        if extension.is_unconnected:
            print(f"    Soma.{soma.uid} <-?-> Ext.{extension.uid}({ep_idx})", end="")
            path, length = cn_.ShortestPathFromToN(
                end_point,
                dijkstra_costs,
                soma.ExtensionPointsCloseTo,
                max_straight_sq_dist=max_straight_sq_dist_c,
            )
            if length <= max_weighted_length_c:
                tgt_extenstion = extension_t.ExtensionContainingSite(extensions, path[-1])
                cn_.ValidateConnection(tgt_extenstion, extension, path, dijkstra_costs)
                should_look_for_connections = True
                print(": Made")
            else:
                print("")

fb_.PrintConnectedExtensions(extensions)
elapsed_time = tm_.gmtime(tm_.time() - start_time)
print(f"\nElapsed Time={tm_.strftime('%Hh %Mm %Ss', elapsed_time)}")
if with_plot:
    fb_.PlotSomasWithExtensions(somas, som_nfo, "with_ext_of_ext")

# -- Summary
print("\n")
for soma in somas:
    print(soma)
# for extension in extensions:
#     print(extension)

# snapshot = tr_.take_snapshot()
# top_file_stats = snapshot.statistics('lineno')
# print("Memory Profiling: Top 10 FILES")
# for stat in top_file_stats[:10]:
#     print(stat)
# top_block_stats = snapshot.statistics('traceback')
# top_block_stats = top_block_stats[0]
# print(f"Memory Profiling: {top_block_stats.count} memory blocks: {top_block_stats.size / 1024:.1f} KiB")
# for line in top_block_stats.traceback.format():
#     print(line)

elapsed_time = tm_.gmtime(tm_.time() - start_time)
print(f"\nElapsed Time={tm_.strftime('%Hh %Mm %Ss', elapsed_time)}")
print(f"DONE: {tm_.strftime('%a, %b %d %Y @ %H:%M:%S')}")

if with_plot:
    pl_.show()

po_.MaximumIntensityProjectionZ(som_nfo['soma_w_ext_lmp'])

# # Skeletonize the somas to extract the graph of the glial cells
# space_dim = 3
# array_shape = (80, 80, 50)
# threshold_fct = lambda img: 0.75 * image.max()
# struct_elm_shape = (3, 3, 3)
#
# soma_w_ext_map = som_nfo['soma_w_ext_lmp']
# skeleton = skl_map_t.FromShapeMap(soma_w_ext_map, store_widths=True)
# skel_graph = skl_graph_t.FromSkeleton(skeleton)
#
# # --- Some info about the skeleton graph
# print(
#     f"Obj map area={np_.count_nonzero(soma_w_ext_map)}\n\n"
#     f"Validity={skel_graph.is_valid}\n\n"
#     f"N nodes={skel_graph.n_nodes}\n"
#     f"N edges={skel_graph.n_edges}\n"
#     f"Highest degree={skel_graph.highest_degree}/{skel_graph.highest_degree_w_nodes}\n\n"
#     f"Length={skel_graph.length}<-{skel_graph.edge_lengths}\n"
#     f"Width=Hom.{skel_graph.reduced_width()}/Het.{skel_graph.heterogeneous_reduced_width()}<-{skel_graph.edge_reduced_widths()}\n"
#     f"Area as WxL={skel_graph.reduced_width() * skel_graph.length}\n"
#     f"Area as WW Length={skel_graph.ww_length}<-{skel_graph.edge_ww_lengths}\n")
#
# # --- Checking correctness of skeleton graph via various rebuilt maps
# rebuilt_skel = skel_graph.RebuiltSkeletonMap()
# part_map_for_check = skeleton.PartMap()
# # Keep next line before its next one
# part_map_for_check[part_map_for_check == skeleton.invalid_n_neighbors] = 0
# part_map_for_check[part_map_for_check > 3] = 3
#
# print(
#     f"Rebuilt skeleton = Original one? Part map? "
#     f"{np_.array_equal(rebuilt_skel > 0, skeleton.map)} "
#     f"{np_.array_equal(rebuilt_skel, part_map_for_check)}")
#
# if space_dim == 3:
#     rebuilt_skel = rebuilt_skel.max(axis=2)
#
## --- Checking correctness of skeleton graph via rebuilt object map
# soma_w_ext_map = soma_w_ext_map.astype(np_.uint8)
# rebuilt_map = skel_graph.RebuiltObjectMap()
#
# if space_dim == 3:
#     soma_w_ext_map = soma_w_ext_map.max(axis=2)
#     rebuilt_map = rebuilt_map.max(axis=2)
#
# # --- Show various images and plot the skeleton graph
# ___, axes = pl_.subplots(1, 3, figsize=(15,15))
# axes[0].matshow(soma_w_ext_map)
# axes[1].matshow(rebuilt_map)
# axes[2].matshow(soma_w_ext_map - rebuilt_map)
# axes[0].set_title("Object")
# axes[1].set_title("Rebuilt")
# axes[2].set_title("Object - Rebuilt")
# for axis in axes:
#     axis.set_axis_off()
# pl_.tight_layout(pad=0.3)
#
# ___, axes = pl_.subplots(1, 3)
# axes[0].matshow(rebuilt_skel + soma_w_ext_map)
# axes[1].matshow(rebuilt_skel + rebuilt_map)
# axes[2].matshow(rebuilt_skel)
# axes[0].set_title("Rebuilt Skel Over Obj")
# axes[1].set_title("Rebuilt Skel Over Rebuilt Obj")
# axes[2].set_title("Rebuilt Skel")
# for axis in axes:
#     axis.set_axis_off()
# pl_.tight_layout(pad=0.3)
#
# if space_dim == 2:
#     skel_graph.Plot(mode=plot_mode_e.Networkx, should_block=False)
#     skel_graph.Plot(mode=plot_mode_e.Graphviz, should_block=False)
#
# skel_graph.Plot(mode=plot_mode_e.SKL_Curve, w_directions=True, should_block=False)
# skel_graph.Plot(should_block=False)
#
# pl_.show()