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dnarXiv
Source_Encoding
Commits
6865c727
Commit
6865c727
authored
2 years ago
by
BOULLE Olivier
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refactoring, size estimations for baa
parent
94946468
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binary_dna_conversion.py
+73
-30
73 additions, 30 deletions
binary_dna_conversion.py
with
73 additions
and
30 deletions
binary_dna_conversion.py
+
73
−
30
View file @
6865c727
...
...
@@ -115,7 +115,7 @@ def dna_to_binary_abaab(sequence: str) -> str:
binary_string
+=
dna_to_bit_pair
[
sequence_rest
[
1
]]
elif
len
(
sequence_rest
)
==
3
:
# a rest of 3 should not occur from a binary to dna abaab conversion, but a non coding
A
can be added to get a round number of blocks
# a rest of 3 should not occur from a binary to dna abaab conversion, but a non coding
base
can be added to get a round number of blocks
binary_string
+=
dna_to_bit_pair
[
sequence_rest
[
1
]]
# just act like the rest was 2
elif
len
(
sequence_rest
)
==
4
:
# 4 bases -> 2 bits + 1 bit + 2 bits + 1 bit
...
...
@@ -188,27 +188,9 @@ def remove_ban_words_abaab_encoding(sequence: str, abaab_method_offset=0) -> str
exit
(
1
)
return
sequence_wo_bans
def
size_binary_from_dna_len_abaab
(
dna_length
):
"""
assume dna_abaab to binary conversion
get the length the binary string will be from the given dna sequence
conversion rate is 1.6bit/base
"""
base_length
=
8
*
(
dna_length
//
5
)
# full conversion of octets
rest
=
dna_length
%
5
if
rest
==
0
:
return
base_length
if
rest
==
1
:
return
base_length
+
2
if
rest
==
2
or
rest
==
3
:
# if rest of 3, act like a rest of 2 because the third base will be ignored
return
base_length
+
4
if
rest
==
4
:
return
base_length
+
6
def
size_
of_
dna_from_bit_len_abaab
(
bit_length
):
def
size_dna_from_bit_len_abaab
(
bit_length
):
"""
assume binary to dna_abaab conversion
get the size the dna sequence will be from the given binary string length
...
...
@@ -226,23 +208,42 @@ def size_of_dna_from_bit_len_abaab(bit_length):
return
base_length
+
4
print
(
"
error size_of_dna_from_bit_len : size not multiple of 2 :
"
,
str
(
bit_length
))
exit
(
1
)
def
size_binary_from_dna_len_abaab
(
dna_length
):
"""
assume dna_abaab to binary conversion
get the length the binary string will be from the given dna sequence
conversion rate is 1.6bit/base
"""
base_length
=
8
*
(
dna_length
//
5
)
# full conversion of octets
rest
=
dna_length
%
5
if
rest
==
0
:
return
base_length
if
rest
==
1
:
return
base_length
+
2
if
rest
==
2
or
rest
==
3
:
# if rest of 3, act like a rest of 2 because the third base will be ignored
return
base_length
+
4
if
rest
==
4
:
return
base_length
+
6
def
binary_to_dna_baa
(
binary_string
:
str
,
GC_window
=
20
)
->
str
:
"""
convert binaries into dna sequence with some properties
the binaries are divided into parts of 5 bits
for each 5 bits part, 1st
2nd bits are normally converted into dna : (00:A, 01:G, 10:T, 11:C)
3rd bit is converted depending on previous converted sequence :
(0:G, 1:C) or (0:A, 1:T), first to break potential homopolymer, if not then to adjust %GC of the previous sequence
4th 5th bits are normally converted into dna
for each 5 bits part, 1st
bit is converted depending on previous converted sequence :
(0:G, 1:C) or (0:A, 1:T), first to break potential homopolymer, if not then to adjust %GC of the local sequence in a defined window
2nd 3rd bits are normally converted into dna : (00:A, 01:G, 10:T, 11:C)
4th 5th bits are normally converted into dna
: (00:A, 01:G, 10:T, 11:C)
this ensure that the total sequence cannot contain homopolymers > 3,
the GC% is in [33%, 66%] in the worst case in a window of 3, but tends to 50% with the adjustments and the conversion rate is 1.66 bit/base
warning : need the binary string to be a multiple of 5, or rest is inconsistent with decoding
ex : a rest of 0 -> A, a rest of 00 -> A
so how to decode A ?
so how to decode
a base
A ?
only allowing rests multiple of 5 removes ambiguity (also possible 0, 1 or 3 modulo 5)
"""
...
...
@@ -400,6 +401,41 @@ def remove_ban_words_baa_encoding(sequence: str, baa_method_offset=0) -> str:
return
sequence_wo_bans
def
size_dna_from_bit_len_baa
(
bit_length
):
"""
assume binary to dna_baa conversion
get the size the dna sequence will be from the given binary string length
conversion rate is 1.66 bit/base
"""
base_length
=
3
*
(
bit_length
//
5
)
# 5 bits -> 3 bases
rest
=
bit_length
%
5
if
rest
==
0
:
return
base_length
if
rest
==
1
:
return
base_length
+
1
if
rest
==
3
:
return
base_length
+
2
print
(
"
error size_of_dna_from_bit_len : invalid rest size:
"
,
str
(
rest
))
exit
(
1
)
def
size_binary_from_dna_len_baa
(
dna_length
):
"""
assume dna_baa to binary conversion
get the length the binary string will be from the given dna sequence
conversion rate is 1.66 bit/base
"""
base_length
=
5
*
(
dna_length
//
3
)
# 3 bases -> 5 bits
rest
=
dna_length
%
3
if
rest
==
0
:
return
base_length
if
rest
==
1
:
return
base_length
+
1
if
rest
==
2
:
return
base_length
+
3
def
test_conversion
():
for
binary_size
in
range
(
100
,
15000
,
1
):
...
...
@@ -417,13 +453,20 @@ def test_conversion():
#decoded_abaab = dna_to_binary_abaab(seq_abaab)
decoded_baa
=
dna_to_binary_baa
(
seq_baa
)
if
size_binary_from_dna_len_baa
(
len
(
seq_baa
))
!=
len
(
random_binary
):
print
(
"
dna
"
,
str
(
len
(
seq_baa
)))
exit
(
0
)
if
size_dna_from_bit_len_baa
(
len
(
random_binary
))
!=
len
(
seq_baa
):
print
(
"
bin
"
,
str
(
len
(
random_binary
)))
exit
(
0
)
#print("check abaab encoding", str(decoded_abaab == random_binary))
print
(
"
check baa encoding
"
,
str
(
decoded_baa
==
random_binary
))
if
not
(
decoded_baa
==
random_binary
and
sequence_control
.
sequence_check
(
seq_baa
,
min_GC
=
40
,
max_GC
=
60
,
verbose
=
True
)):
#
print("check baa encoding", str(decoded_baa == random_binary))
"""
if not (decoded_baa == random_binary and sequence_control.sequence_check(seq_baa, min_GC=40, max_GC=60, verbose=True)):
#print(random_binary)
print(seq_baa)
#print(decoded_baa)
exit
(
1
)
exit(1)
"""
#print("check abaab encoding")
#sequence_control.sanity_check(seq_abaab)
...
...
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