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vidjil
vidjil
Commits
c68f3ac0
Commit
c68f3ac0
authored
Jul 25, 2014
by
Mikaël Salson
Browse files
Doc: Uniformisation of quotes in manual
parent
9e510e43
Changes
1
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c68f3ac0
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@@ -17,18 +17,18 @@ The Vidjil browser runs in any modern browser. It has been successfully tested o
** First aid
- Go to the
«
file
»
menu to access your data.
- Go to the
“
file
”
menu to access your data.
Your files are protected with your login and password.
There is always a
«
sample/L2-LIL.data
»
dataset for demonstration purposes.
There is always a
“
sample/L2-LIL.data
”
dataset for demonstration purposes.
- You can change the number of displayed clones by moving the slider
«
number of clones
»
(menu
«
filter
»
)
- You can change the number of displayed clones by moving the slider
“
number of clones
”
(menu
“
filter
”
)
The maximal number of clones that can be displayed depends on what has been run before (by default, 200) XXX
- Due to sequencing errors, there may be several clones corresponding to a real clone.
- You can select several clones (for example those sharing a same V and a same J),
- inspect the sequences in the lower panel (possibly using the
«
align
»
function)
- click on
«
merge
»
if you think that the clones should be merged.
Once several clones are merged, you can still visualize them by clicking on
« + »
in the list of clones.
- inspect the sequences in the lower panel (possibly using the
“
align
”
function)
- click on
“
merge
”
if you think that the clones should be merged.
Once several clones are merged, you can still visualize them by clicking on
“+”
in the list of clones.
* The elements of the Vidjil browser
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...
@@ -45,13 +45,13 @@ The Vidjil browser runs in any modern browser. It has been successfully tested o
** The list of clones (left panel)
- You can assign other tags (and thus colors) to clones using the
«
star
»
button.
The
«
filter
»
menu allows to further filter clones by tags.
- Under the
«
star
»
button it is possible to normalise clone concentrations
- You can assign other tags (and thus colors) to clones using the
“
star
”
button.
The
“
filter
”
menu allows to further filter clones by tags.
- Under the
“
star
”
button it is possible to normalise clone concentrations
according to this clone. You must specify the expected concentration in the
“expected size” textfield (e.g. 0.01 for 1%).
- The
« i »
button displays additional information on each clone
- The
“i”
button displays additional information on each clone
- The list can be sorted on V genes, J genes or concentrations. At the top of
the list you need to click respectively on “V sort”, “J sort” or “sort”.
...
...
@@ -62,19 +62,19 @@ The Vidjil browser runs in any modern browser. It has been successfully tested o
- The gray areas at the bottom of the graph show, for each point, the resolution (1 read / 5 reads)
- You can reorder the points by dragging them, and hide some points by dragging them on the
"+"
mark at the right of the points.
- You can reorder the points by dragging them, and hide some points by dragging them on the
“+”
mark at the right of the points.
If you want to recover some hidden points, you need to drag them from the “+” mark to the graph.
- If your dataset contains sampling dates (for example in a MRD setup), you can switch between point keys and dates in
"
settings > point key
"
- If your dataset contains sampling dates (for example in a MRD setup), you can switch between point keys and dates in
“
settings > point key
”
- The vertical gray area shows the current point, you can change that by clicking on another point.
** The scatterplot view
- The axes of the plot (by default
«
V gene
»
/
«
J gene
»
) can be changed
- The axes of the plot (by default
“
V gene
”
/
“
J gene
”
) can be changed
- Some presets are available in the
«
analysis
»
menu.
- Some presets are available in the
“
analysis
”
menu.
To segregate a set of clones sharing a same V and J, it is often useful
to display the clones according to their 'N length' (that is N1-D-N2 in the cas of VDJ rearrangements)
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@@ -97,7 +97,7 @@ The Vidjil browser runs in any modern browser. It has been successfully tested o
To make sure that the PCR, the sequencing and the Vidjil analysis went well, several elements can be controlled.
** Number of segmented reads
A first way is to check the number of
«
segmented reads
»
in the info panel. For each point, this shows the number of reads where Vidjil found a CDR3.
A first way is to check the number of
“
segmented reads
”
in the info panel. For each point, this shows the number of reads where Vidjil found a CDR3.
Ratios above 90% usually mean very good results. Smaller ratios, especially under 60%, often mean that something went wrong.
There can be several causes leading to bad ratios:
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@@ -144,7 +144,7 @@ There can be several causes leading to bad ratios:
If you use Vidjil for your research, please cite the following reference:
Mathieu Giraud, Mikaël Salson, et al.,
"
Fast multiclonal clusterization of V(D)J recombinations from high-throughput sequencing
"
,
“
Fast multiclonal clusterization of V(D)J recombinations from high-throughput sequencing
”
,
BMC Genomics 2014, 15:409
http://dx.doi.org/10.1186/1471-2164-15-409
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