Commit 945e896e authored by Mathieu Giraud's avatar Mathieu Giraud
Browse files help on -r/-R/-%/-z/-A

parent 404dbc33
......@@ -108,10 +108,56 @@ Setting =-w= to 30 for TRG and 50 for IGH may "segment" (analyze) a
few more reads, but may in some rare cases falsely cluster reads from
different clones. Setting =-w= to lower values is not recommended.
** Threshold on clone output
The following options control how many clones are output and analyzed.
Limit to keep a window
-r <nb> minimal number of reads containing a window (default: 10)
Limits to report a clone
-R <nb> minimal number of reads supporting a clone (default: 10)
-% <ratio> minimal percentage of reads supporting a clone (default: 0)
Limits to segment a clone
-z <nb> maximal number of clones to be segmented (0: no limit, do not use) (default: 20)
-A reports and segments all clones (-r 0 -R 1 -% 0 -z 0), to be used only on very small datasets
The =-r/-R/-%= options are strong thresholds: if a clone does not have
the requested number of reads, the clone is discarded (except when
using =-l=, see below).
The =-r= option is applied before the additional clusterization, the
=-R/-%= options after it.
The default =-r 10 -R 10= options are meant to only output clones that
have a significant read support. You can safely put =-r 1 -R 1= if you
want to detect all clones starting from the first read (especially for
MRD detection).
The =-z= option limits the number of clones that are fully analyzed,
/with their V(D)J segmentation/, in particular to enable the browser
to display the clones on the grid (otherwise they are displayed on the
'?/?' axis).
If you want have to analyze more clones, you should use =-z 50= or
=-z 100=. It is not recommended to use larger values: outputing more
than 100 clones is often not useful to visualize browser
visualization, and takes large computation time.
Note that even if a clone is not in the top 20 (or 50, or 100) but
still passes the =-R=, =-%= options, it is still reported in the .data
file. If the clone is at some MRD point in the top 20 (or 50, or 100),
it will be fully analyzed/segmented by this other point (and the
collected by the script, and then, on the browser, correctly
displayed on the grid).
The =-A= option disables all these thresholds. This option should be
used only for test and debug purposes, on very small datasets, and
produce large file and takes huge computation times.
** Force to follow some sequences
Vidjil allows to specify a list of windows that must be followed
(even if those windows are 'rare', below the -r/-R/-% thresholds).
(even if those windows are 'rare', below the =-r/-R/-%= thresholds).
The parameter =-l= is made for providing such a list in a file following
the following format: window label (separed by one space)
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