Skip to content
GitLab
Menu
Projects
Groups
Snippets
Loading...
Help
Help
Support
Community forum
Keyboard shortcuts
?
Submit feedback
Contribute to GitLab
Sign in
Toggle navigation
Menu
Open sidebar
vidjil
vidjil
Commits
5ab5f48e
Commit
5ab5f48e
authored
Apr 27, 2015
by
Mikaël Salson
Browse files
browser.org: Documentation on clone coverage
parent
e6b51cf0
Changes
1
Hide whitespace changes
Inline
Side-by-side
doc/browser.org
View file @
5ab5f48e
...
@@ -220,7 +220,7 @@ analyzed reads, including the hidden clones.
...
@@ -220,7 +220,7 @@ analyzed reads, including the hidden clones.
* What is the sequence displayed for each clone ?
* What is the sequence displayed for each clone ?
<<representative>>
The sequences displayed for each clone are not individual reads.
The sequences displayed for each clone are not individual reads.
The clones may gather thousands of reads, and all these reads can have
The clones may gather thousands of reads, and all these reads can have
some differences. Depending on the sequencing technology, the reads
some differences. Depending on the sequencing technology, the reads
...
@@ -292,7 +292,21 @@ There can be several causes leading to bad ratios:
...
@@ -292,7 +292,21 @@ There can be several causes leading to bad ratios:
spot on the graph any large change in concentration.
spot on the graph any large change in concentration.
** Clone coverage
The clone coverage is computed over the consensus sequence which is
displayed for each clone (see [[representative][What is the sequence displayed for each clone?]]).
Its length should be representative of the read lengths among that clone. A
clone can be constituted of thousands of reads of various lengths. We
expect the consensus sequence to be close to the median read length of the
clone. The clone coverage is such a measure: having a clone coverage
between .85 and 1 is quite frequent. On the contrary, if it is .5 it means that the consensus sequence
length is half shorter than the median read length in the clone.
There is a bad clone coverage ($<.5$) when reads do share the same window
(it is how Vidjil defines a clone) and when they have frequent discrepancies
outside of the window. Such cases have been observed with chimeric reads
which share the same V(D)J recombinations in their first half and have
totally different and unknown sequences in their second half.
* Keyboard shortcuts
* Keyboard shortcuts
** Browser
** Browser
...
...
Write
Preview
Markdown
is supported
0%
Try again
or
attach a new file
.
Attach a file
Cancel
You are about to add
0
people
to the discussion. Proceed with caution.
Finish editing this message first!
Cancel
Please
register
or
sign in
to comment