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vidjil
vidjil
Commits
080ed75a
Commit
080ed75a
authored
Nov 26, 2014
by
Mathieu Giraud
Browse files
algo.org: remove '-d' and '-R' from examples
These options do not exist anymore.
parent
31b79a96
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doc/algo.org
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080ed75a
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@@ -145,9 +145,9 @@ Window prediction
(default: #####-#####, ######-######, #######-#######, depends on germline)
-k <int> k-mer size used for the V/J affectation (default: 10, 12, 13, depends on germline)
(using -k option is equivalent to set with -s a contiguous seed with only '#' characters)
-m <int> minimal admissible delta between last V and first J k-mer (default: -10) (default with -
d
: 0)
-M <int> maximal admissible delta between last V and first J k-mer (default: 20) (default with -
d
: 80)
-w <int> w-mer size used for the length of the extracted window (default: 40)(default with -
d
: 60)
-m <int> minimal admissible delta between last V and first J k-mer (default: -10) (default with -
D
: 0)
-M <int> maximal admissible delta between last V and first J k-mer (default: 20) (default with -
D
: 80)
-w <int> w-mer size used for the length of the extracted window (default: 40)(default with -
D
: 60)
#+END_EXAMPLE
The =-s=, =-k=, =-m= and =-M= options are the options of the seed-based heuristic. A detailed
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@@ -252,10 +252,10 @@ two windows that must be clustered.
* Examples of use
All the following examples are on a IGH VDJ recombinations : they thus
require the =-G germline/IGH=
and the =-d
= option
s
.
require
either
the =-G germline/IGH=
option, or the mutli-germline =-g germline
= option.
#+BEGIN_SRC sh
./vidjil -G germline/IGH
-d
data/Stanford_S22.fasta
./vidjil -G germline/IGH data/Stanford_S22.fasta
# Extract (with an ultra-fast heuristic) all windows
# Summary of windows is available both in out/Stanford_S22.vdj.fa
# and in out/Stanford_S22.vidjil.
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...
@@ -273,7 +273,7 @@ CTATGATAGTAGTGGTTATTACGGGGTAGGGCAGTACTACTACTACTACATGGACGTCTG
The first window has 8 occurrences, the second window has 5 occurrences.
#+BEGIN_SRC sh
./vidjil -c clones -G germline/IGH -r 1
-R 1 -d
./data/clones_simul.fa
./vidjil -c clones -G germline/IGH -r 1 ./data/clones_simul.fa
# Extracts the windows (-r 1, with at least 1 read each),
# then gather them into clones
# A more natural option could be -r 5.
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@@ -288,13 +288,13 @@ CTATGATAGTAGTGGTTATTACGGGGTAGGGCAGTACTACTACTACTACATGGACGTCTG
#+END_SRC
#+BEGIN_SRC sh
./vidjil -c clones -G germline/IGH -r 1 -n 5
-d
./data/clones_simul.fa
./vidjil -c clones -G germline/IGH -r 1 -n 5 ./data/clones_simul.fa
# Window extraction + clone gathering,
# with automatic clustering, distance five (-n 5)
#+END_SRC
#+BEGIN_SRC sh
./vidjil -c segment -G germline/IGH
-d
data/segment_S22.fa
./vidjil -c segment -G germline/IGH data/segment_S22.fa
# Segment the reads onto VDJ germline
# (this is slow and should only be used for testing)
#+END_SRC
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